UniProt AC | UniProt Status | Protein Name | Organism | PMID | Article Title | Category | Annotation | Contributor | ORCID | Submission Date | |
P69905 | Reviewed | Hemoglobin subunit alpha | Homo sapiens (Human). | 2594814 | Transcriptional regulatory factors may control the relative expression of human alpha 1 and alpha 2-globin genes in erythroleukemia cells. | [Expression][Sequence] | Protein/gene_name: Hemoglobin subunit alpha. Disease: Alpha thalassemia. Additional Annotation: Transcriptional regulatory factors may control the relative expression of human alpha 1 and alpha 2-globin genes in erythroleukemia cells. | Nicholas Moschonas | 0000-0002-2556-537X | 2022-5-15 | |
P69905 | Reviewed | Hemoglobin subunit alpha | Homo sapiens (Human). | 2227935 | Locus assignment of human alpha-globin structural mutants by selective enzymatic amplification of alpha 1 and alpha 2-globin cDNAs. | [Pathology & Biotech][Sequence] | Protein/gene_name: Hemoglobin subunit alpha. Disease: Alpha thalassemia. Additional Annotation: The selective assignment of human alpha-globin structural mutants to one of the two highly homologous alpha-globin genes is proposed. Selectively amplified alpha 1 and alpha 2-globin cDNAs by DNA enzymatic amplification were dot-blotted and further hybridized to synthetic oligonucleotides encompassing either the normal or the mutated sequences. The generated signals corresponded specifically to one of the two alpha-globin genes. Using this approach the alpha-globin structural mutants J-Buda and G-Pest were found to be encoded by the alpha 2 and the alpha 1-globin genes, respectively. Furthermore, the exact nucleotide changes were determined. | Nicholas Moschonas | 0000-0002-2556-537X | 2022-5-15 | |
P69905 | Reviewed | Hemoglobin subunit alpha | Homo sapiens (Human). | 2117325 | Aberrance and modification of alpha-1- and alpha-2-globin gene expression in human and mouse erythroleukemia cells. | [Expression] | Protein/gene_name: alpha-1 globin; alpha-2 globin. Disease: Alpha thalassemia. Additional Annotation: In contrast to normal human erythroid tissues where the alpha 2:alpha 1-globin mRNA ratio is about 72:28, in human erythroleukemia K562 cells this ratio was found to be quite low, i.e. about 8:92. The ratio was moderately increased by hemin induction and approached almost normal levels after chromosomal transfer from K562 to the mouse erythroleukemia (MEL) cells. We suggest that operationally positive regulatory factors may exist in erythroleukemia cells, modifying the relative alpha 1- and alpha 2-globin gene expression by events following induction and by the adult MEL environment. These factors may act by altering the relative rate of alpha 1- and alpha 2-globin mRNA synthesis. | Nicholas Moschonas | 0000-0002-2556-537X | 2022-5-15 |