UniProt AC | UniProt Status | Protein Name | Organism | PMID | Article Title | Category | Annotation | Contributor | ORCID | Submission Date | |
Q92622 | Reviewed | Run domain Beclin-1-interacting and cysteine-rich domain-containing protein | Homo sapiens (Human). | 32632011 | Structural basis for autophagy inhibition by the human Rubicon-Rab7 complex. | [Function][Pathology & Biotech][Expression][Interaction][Structure] | Protein/gene_name: Rubicon; RUBCN. Function: Interacts with GTP-bound RAB7. Involved in negative regulation of autophagy. Additional Annotation: 2.8-angstrom crystal structure of the Rubicon RH domain in complex with RAB7-GTP. The structure reveals a fold for the RH domain built around four zinc clusters. The switch regions of RAB7 insert into pockets on the surface of the RH domain in a mode that is distinct from those of other Rab-effector complexes. Rubicon residues at the dimer interface are required for Rubicon and RAB7 to colocalize in living cells. Mutation of Rubicon RH residues in the Rab7-binding site restores efficient autophagic flux in the presence of overexpressed Rubicon. | Hersh K. Bhargava | 0000-0003-4675-9293 | 2020-9-28 |